Part:BBa_K1088018:Experience

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Applications of BBa_K1088018

In Synechocystis it has been suggested that the amount of LacI present is correlated to the leakiness of LacI dependent promoters (Albers et al. 2015). It has also been reported that the addition of the highly active LVA tag to proteins greatly decreases protein concentration (Wang et al. 2012). It is because of this that the synthesis of a LacI without an LVA tag was decided.

Edinburgh_OG 2016 decided to improve this part (New part BBa_K1968024) in order to adjust to the Phytobrick standard. Two BbsI sites and one BsmbI site had to be removed (Codons in the basepairs 494-496, 833-835 were changed from GAT to GAC, the codon in basepair 962-964 was changed from GTC to GTG maintaining the same aminoacid sequence and making it Phytobrick compatible).

Albers, S.C., Gallegos, V.A. & Peebles, C.A.M., 2015. Engineering of genetic control tools in Synechocystis sp. PCC 6803 using rational design techniques. Journal of Biotechnology, 216, pp.36–46. Andreassen, P.R. et al., 2014. Natural LacI from E. coli Yields Faster Response and Higher Level of Expression than the LVA-Tagged LacI. ACS Synthetic Biology, 3(12), pp.949–952.

Wang, B. et al., 2012. Application of synthetic biology in cyanobacteria and algae. Frontiers in Microbiology, 3, p.344.

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